Alleviation of allergic conjunctivitis by (±)5(6)-dihydroxy-8Z,11Z,14Z,17Z-eicosatetraenoic acid in mice

Background: Allergic conjunctivitis (AC) is a common ophthalmologic disorder that causes symptoms often reduces patient’s quality of life (QOL). We investigated the effects eicosapentaenoic acid metabolite (±)5(6)-dihydroxy-8Z,11Z,14Z,17Z-eicosatetraenoic ((±)5(6)-DiHETE) on AC using mouse model. Methods: BALB/c mice were sensitized with two injections short ragweed pollen in alum, challenged fifth eyedrops. The clinical signs and tear volume evaluated at 15 min after final challenge. Histamine-induced ocular inflammation model was prepared by instilling histamine onto surface eye. Fifteen minutes application, measured Schirmer test. Miles assay performed to investigate vascular permeability. To cause scratching behavior 10 μg serotonin injected cheek. Results: Repeated topical application induced conjunctivitis, accompanied eyelid edema tearing mice. Pollen typically degranulates mast cells recruits eosinophils conjunctiva. Intraperitoneal administration 300 μg/kg (±)5(6)-DiHETE significantly inhibited pollen-induced symptoms. also attenuated cell degranulation eosinophil infiltration into assess downstream pathway activation AC, we used histamine-induced Topical 4 μg/eye caused increased permeability, as indicated Evans blue dye extravasation. or 1 manifestations. Finally, assessed itching. An intradermal injection cheek serotonin-induced scratching. Conclusion: Thus, treatment broadly suppressed pathology could be novel option for AC.